Notably, BME contains 13 essential amino acids and nine vitamins. BME is a type of artificial media developed by Harry Eagle in Also, it was used on mouse L cells and human HeLa cells. Significantly, it contains 13 amino acids and 9 vitamins. It makes the refeeding time of the culture longer. Also, he removed biotin vitamin B 7 , one of the nine vitamins of the BME, which is superfluous. Similarly, it contains two or four times more glucose in comparison to EMEM. In addition to these, it contains iron, which comes from ferric sulfate.
Thus, it makes DMEM is more suitable for specific cell types. However, as DMEM is a basal medium, it does not contain proteins or growth-promoting agents. It is a simple, basal media with the minimum amounts of nutrient compositions.
The key difference between two media is the nutrient composition. EMEM is composed of the minimum concentrations of nutrients required for the growth of the culture, whereas DMEM is a much complex media with increased concentrations of amino acids and vitamins. Overview and Key Difference 2. What is DMEM 3. What is EMEM 4. This is adapted from EMEM, and the nutrient composition is modified by increasing the concentration of amino acids and vitamins.
The concentration of amino acids is increased up to two-fold in comparison to the basal medium. The vitamin concentration is increased up to fourfold thereby increasing the nutrient content in the medium. DMEM is also modified by adding more salts such as ferric nitrate, sodium pyruvate and some non — essential amino acids such as serine and glycine. The concentration of glucose in the media is also altered. DMEM also requires supplementation of a serum medium as it is not a complete medium. FBS provides the required proteins and growth factors for the culturing process.
The pH of the medium varies upon addition of Sodium Bicarbonate. The storage temperature of the media is 2 — 8 0 C. The EMEM media is also a type of modified media. EMEM media included the essential amino acids and vitamins in a minimum concentration required by the cell types. Amino acid concentrations conform closely to the protein composition of human cells. Higher concentrations of nutrients permit longer periods between feedings. Related Products: Hanks. EMEM is suitable for culturing a broad spectrum.
Related Products: Fetal Bovine Serum. Related Products: 6. This antibody has been tested for use in western blotting, immunofluorescence microscopy and immunoelectron microscopy. Specific conditions for reactivity should be optimized by the end user. This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot.
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